6AC9
Crystal structure of human Vaccinia-related kinase 1 (VRK1) in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-19 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.596, 96.846, 192.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.070 |
R-factor | 0.187 |
Rwork | 0.186 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.000 | 2.110 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.106 | 0.701 |
Number of reflections | 106113 | 5210 |
<I/σ(I)> | 21.3 | 5.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 22.4 | 22.5 |
CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 27.5 % w/v PEG 3350, 0.2 M of ammonium sulfate, 0.1 M of HEPES (pH 7.0) |