6ABU
Rat Xanthine oxidoreductase, NAD bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.353, 137.663, 221.969 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.570 - 1.770 |
R-factor | 0.20083 |
Rwork | 0.199 |
R-free | 0.23661 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wyg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.506 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.570 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.117 | 1.672 |
Number of reflections | 291681 | |
<I/σ(I)> | 1.34 | |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 8000, HEPES, lithium formate, pH 6.2 |