6ABT
Crystal structure of transcription factor from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2017-12-17 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 1.0003 |
Spacegroup name | P 64 |
Unit cell lengths | 67.024, 67.024, 90.288 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.040 - 2.800 |
R-factor | 0.2573 |
Rwork | 0.255 |
R-free | 0.28830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6abq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.627 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.040 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.080 | 0.051 | 0.334 |
Rmeas | 0.086 | 0.056 | 0.360 |
Rpim | 0.033 | 0.022 | 0.133 |
Number of reflections | 5721 | 295 | 266 |
<I/σ(I)> | 11.9 | ||
Completeness [%] | 99.9 | 97.4 | 100 |
Redundancy | 7.3 | 6.1 | 7.3 |
CC(1/2) | 0.995 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | with 0.2 M calcium acetate, 0.1 M cacodylate acid, pH 6.3, and 48 % polyethylene glycol 600 |