6ABS

Actin interacting protein 5 (Aip5, mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-31
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.942, 54.596, 128.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.272 - 2.200
R-factor	0.2023
Rwork	0.197
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6abr
RMSD bond length	0.008
RMSD bond angle	1.119
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	14417	699
<I/σ(I)>	14
Completeness [%]	98.3
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium phosphate citrate pH 4.2, 40% PEG300