6AB9
The crystal structure of the relaxed state of Nonlabens marinus Rhodopsin 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.634, 49.220, 69.327 |
| Unit cell angles | 90.00, 110.02, 90.00 |
Refinement procedure
| Resolution | 33.636 - 1.750 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g28 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.004 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.636 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.061 | |
| Number of reflections | 32559 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.2 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 293 | 30% polyethylene glycol dimethyl ether 500, 0.1 M sodium chloride, 0.1 M MES (pH 6.0) |
