6AAY
the Cas13b binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 196.740, 196.740, 96.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.020 - 2.790 |
| R-factor | 0.22393 |
| Rwork | 0.221 |
| R-free | 0.27613 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.415 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.580 | 2.890 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Number of reflections | 47521 | 2376 |
| <I/σ(I)> | 12.2 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20 | |
| CC(1/2) | 0.997 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.15 M Sodium chloride, 0.1 M Sodium citrate (pH 5.5), 22% (w/v) Polyethylene glycol 1,000 |
