6AAP
Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with ZaeSeCys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.797, 43.609, 72.478 |
| Unit cell angles | 90.00, 119.67, 90.00 |
Refinement procedure
| Resolution | 37.041 - 1.500 |
| R-factor | 0.1651 |
| Rwork | 0.164 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zim |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.053 | |
| Rpim | 0.010 | 0.138 |
| Number of reflections | 43994 | 2174 |
| <I/σ(I)> | 85 | |
| Completeness [%] | 99.7 | |
| Redundancy | 29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | Tris-HCl (pH 8.5), PEG 200, KCl, MgCl2, sodium citrate |
