6AAF
Crystal structure of fission yeast Atg8 complexed with the helical AIM of Hfl1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-11-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 33.798, 108.866, 35.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.120 - 2.197 |
| R-factor | 0.2161 |
| Rwork | 0.211 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zpn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.730 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.120 | 2.330 |
| High resolution limit [Å] | 2.197 | 2.197 |
| Rmerge | 0.122 | 1.331 |
| Number of reflections | 7091 | 1101 |
| <I/σ(I)> | 8.1 | 1.1 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 27.5% PEG8000, 0.2 M sodium acetate, 0.1 M Bis-Tris pH 5.5 |
