6AAE
Crystal structure of Chloramphenicol-Metabolizaing Enzyme EstDL136
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 118.943, 153.586, 44.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.258 - 1.641 |
R-factor | 0.1899 |
Rwork | 0.189 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u4n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.068 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 100067 | |
<I/σ(I)> | 23 | |
Completeness [%] | 100.0 | |
Redundancy | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 500mM Ammonium fluoride (pH6.5), 30% PEG3350, 5% glycerol and 120mM TCEP |