6AA7
Fluorescent protein from Acropora digitifera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.618, 90.553, 73.429 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.170 - 1.800 |
R-factor | 0.17811 |
Rwork | 0.177 |
R-free | 0.20752 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y8q |
RMSD bond length | 0.018 |
RMSD bond angle | 2.023 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.067 | 0.375 |
Rmeas | 0.071 | 0.375 |
Rpim | 0.023 | 0.140 |
Number of reflections | 49007 | 2426 |
<I/σ(I)> | 33.05 | 2.88 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 8.1 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.5 | HEPES, Lithium sulfate |