6AA0
Crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in Apo Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 300 |
Detector technology | PIXEL |
Collection date | 2017-11-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.980066 |
Spacegroup name | P 31 |
Unit cell lengths | 113.070, 113.070, 199.813 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 69.932 - 3.200 |
R-factor | 0.1899 |
Rwork | 0.188 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xif |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX (1.15rc2_3428) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.910 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 47193 | 3515 |
<I/σ(I)> | 10.3 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 10.7 |
CC(1/2) | 0.996 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | Published |