6A9Y

The crystal structure of Mu homology domain of SGIP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	70.073, 132.308, 40.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.912 - 2.700
R-factor	0.2017
Rwork	0.198
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5awu
RMSD bond length	0.008
RMSD bond angle	1.313
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.970
High resolution limit [Å]	2.700	2.700
Number of reflections	10898	565
<I/σ(I)>	35.3
Completeness [%]	99.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M Tris, pH 8.0, 3.0 M NaCl