6A9W

Structure of the bifunctional DNA primase-polymerase from phage NrS-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	119.874, 57.570, 47.631
Unit cell angles	90.00, 94.03, 90.00

Refinement procedure

Resolution	38.540 - 1.800
R-factor	0.1803
Rwork	0.178
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.540	38.540	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.077	0.051	1.042
Rmeas	0.078	0.052	1.057
Rpim	0.013	0.010	0.173
Number of reflections	30021	212	1758
<I/σ(I)>	32.8
Completeness [%]	99.7	80.4	99.5
Redundancy	36.8	29.8	36.8
CC(1/2)	1.000	1.000	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	100mM Bis-Tris, pH 6.5, 0.2M Li2SO4, 25% PEG 3350