6A9V

Crystal structure of Icp55 from Saccharomyces cerevisiae (N-terminal 42 residues deletion)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-02-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97947
Spacegroup name	I 4 2 2
Unit cell lengths	142.081, 142.081, 118.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.554 - 2.900
R-factor	0.2137
Rwork	0.213
R-free	0.22510
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.554	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.157	1.060
Rmeas	0.166	1.125
Rpim	0.056	0.374
Number of reflections	13770	2173
<I/σ(I)>	15.2	2.5
Completeness [%]	100.0	100
Redundancy	8.7	8.9
CC(1/2)	0.997	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	100mM HEPES pH 7.5, 22 % Peg3350 , 250mM Ammonium Sulphate, 0.2mM MnCl2