6A9T

Crystal structure of Icp55 from Saccharomyces cerevisiae (N-terminal 58 residues deletion)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	I 4 2 2
Unit cell lengths	141.163, 141.163, 118.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.727 - 2.150
R-factor	0.1891
Rwork	0.187
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a16
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.350	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.081	0.844
Rmeas	0.087	0.920
Rpim	0.031	0.357
Number of reflections	31124	1901
<I/σ(I)>	23.1	2.1
Completeness [%]	95.3	68.9
Redundancy	7.7	6.1
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7	293	100 mM HEPES, 30% Jeffamine ED2001, pH 7.0, 0.2 mM MnCl2