6A9P
Crystal structure of the human glial fibrillary acidic protein 1B domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-06-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 23.588, 106.158, 113.225 |
| Unit cell angles | 77.56, 90.14, 83.80 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| R-factor | 0.27071 |
| Rwork | 0.269 |
| R-free | 0.31086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uf1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.614 |
| Number of reflections | 29465 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 94.9 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 100 mM Tris-HCl pH 8.0, 400 mM NaCl and 22% (w/v) PEG10,000 |
