6A9J

Crystal structure of the PE-bound N-terminal domain of Atg2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	86.657, 62.861, 86.662
Unit cell angles	90.00, 91.89, 90.00

Refinement procedure

Resolution	38.233 - 2.700
R-factor	0.2555
Rwork	0.251
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a9e
RMSD bond length	0.003
RMSD bond angle	0.863
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.381	0.439
Number of reflections	25921
<I/σ(I)>	23.8
Completeness [%]	99.9
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG400, ammonium sulfate, Bis-Tris