6A9E
Crystal structure of the N-terminal domain of Atg2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-03-08 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9692 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.451, 62.695, 86.317 |
| Unit cell angles | 90.00, 91.83, 90.00 |
Refinement procedure
| Resolution | 44.103 - 3.205 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.26640 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.145 | 0.579 |
| Number of reflections | 29622 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG400, ammonium sulfate, Bis-Tris |
