6A9D

Crystal structure of the strigolactone receptor ShHTL7 from Striga hermonthica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9800
Spacegroup name	C 1 2 1
Unit cell lengths	92.548, 92.500, 75.428
Unit cell angles	90.00, 116.92, 90.00

Refinement procedure

Resolution	39.286 - 2.302
R-factor	0.186
Rwork	0.183
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cbk
RMSD bond length	0.008
RMSD bond angle	1.169
Data reduction software	SHELX
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.098	0.066	0.386
Rmeas	0.118	0.079	0.462
Rpim	0.065	0.043	0.252
Total number of observations	83014
Number of reflections	25025	1282	1259
<I/σ(I)>	6.6
Completeness [%]	99.6	98.8	99.5
Redundancy	3.3	3.3	3.2
CC(1/2)		0.990	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl pH 8.5, 0.2M MgCl2, 20% PEG 3350