6A9D
Crystal structure of the strigolactone receptor ShHTL7 from Striga hermonthica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.548, 92.500, 75.428 |
| Unit cell angles | 90.00, 116.92, 90.00 |
Refinement procedure
| Resolution | 39.286 - 2.302 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cbk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.169 |
| Data reduction software | SHELX |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.098 | 0.066 | 0.386 |
| Rmeas | 0.118 | 0.079 | 0.462 |
| Rpim | 0.065 | 0.043 | 0.252 |
| Total number of observations | 83014 | ||
| Number of reflections | 25025 | 1282 | 1259 |
| <I/σ(I)> | 6.6 | ||
| Completeness [%] | 99.6 | 98.8 | 99.5 |
| Redundancy | 3.3 | 3.3 | 3.2 |
| CC(1/2) | 0.990 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 0.2M MgCl2, 20% PEG 3350 |
