6A92
Crystal structure of a cyclase Filc1 from Fischerella sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-02 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.519, 255.388, 36.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.680 - 1.580 |
| R-factor | 0.12763 |
| Rwork | 0.125 |
| R-free | 0.18032 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yvk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.640 |
| High resolution limit [Å] | 1.570 | 3.400 | 1.570 |
| Rmerge | 0.060 | 0.036 | 0.513 |
| Rmeas | 0.063 | 0.037 | 0.537 |
| Rpim | 0.017 | 0.011 | 0.158 |
| Total number of observations | 1438922 | ||
| Number of reflections | 114161 | 11877 | 11202 |
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.8 | 98.8 | 99.8 |
| Redundancy | 12.7 | 11.6 | 11.1 |
| CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.0 M sodium citrate, 0.1 M Imidazole pH 8.0 |
