6A8X

Crystal structure of a apo form cyclase from Fischerella sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-02
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	90.993, 90.993, 234.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.850 - 2.350
R-factor	0.19692
Rwork	0.195
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yvk
RMSD bond length	0.010
RMSD bond angle	1.380
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.430
High resolution limit [Å]	2.350	5.050	2.350
Rmerge	0.077	0.032	0.706
Rmeas	0.082	0.035	0.745
Rpim	0.028	0.012	0.231
Total number of observations	362774
Number of reflections	40767	4242	4121
<I/σ(I)>	12.2
Completeness [%]	96.8	93.6	100
Redundancy	8.9	7.7	10.1
CC(1/2)		0.999	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	1.0M sodium citrate, 0.1M Imidazole pH 8.0