6A8X
Crystal structure of a apo form cyclase from Fischerella sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-02 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.993, 90.993, 234.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.850 - 2.350 |
| R-factor | 0.19692 |
| Rwork | 0.195 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yvk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.050 | 2.350 |
| Rmerge | 0.077 | 0.032 | 0.706 |
| Rmeas | 0.082 | 0.035 | 0.745 |
| Rpim | 0.028 | 0.012 | 0.231 |
| Total number of observations | 362774 | ||
| Number of reflections | 40767 | 4242 | 4121 |
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 96.8 | 93.6 | 100 |
| Redundancy | 8.9 | 7.7 | 10.1 |
| CC(1/2) | 0.999 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.0M sodium citrate, 0.1M Imidazole pH 8.0 |
