6A8P

Transglutaminase 2 mutant G224V in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	C 2 2 21
Unit cell lengths	133.195, 216.312, 166.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.271 - 2.537
R-factor	0.2371
Rwork	0.236
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pyg
RMSD bond length	0.007
RMSD bond angle	1.235
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.271	2.566
High resolution limit [Å]	2.537	2.537
Number of reflections	78019
<I/σ(I)>	19.8
Completeness [%]	99.6
Redundancy	13
CC(1/2)	0.999	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	20 mM Mes (pH 6.8), 200 mM sodium chloride, 20 mM MgCl2, 6% PEG 3350, 5 mM DTT, 24% glycerol