6A8M
N-terminal domain of FACT complex subunit SPT16 from Eremothecium gossypii (Ashbya gossypii)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-03-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97947 |
Spacegroup name | H 3 |
Unit cell lengths | 119.885, 119.885, 76.662 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.023 - 1.700 |
R-factor | 0.1688 |
Rwork | 0.167 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ce6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.895 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.037 | 0.467 |
Rmeas | 0.045 | 0.579 |
Rpim | 0.026 | |
Number of reflections | 43344 | 2384 |
<I/σ(I)> | 21.3 | 2.2 |
Completeness [%] | 95.0 | 98.1 |
Redundancy | 2.7 | 2.6 |
CC(1/2) | 0.999 | 0.692 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.9 | 294.15 | 0.2M potassium nitrate, pH 6.9, 20% polyethylene glycol 3350 |