6A8M

N-terminal domain of FACT complex subunit SPT16 from Eremothecium gossypii (Ashbya gossypii)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-03-20
Detector	MARRESEARCH
Wavelength(s)	0.97947
Spacegroup name	H 3
Unit cell lengths	119.885, 119.885, 76.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.023 - 1.700
R-factor	0.1688
Rwork	0.167
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ce6
RMSD bond length	0.007
RMSD bond angle	0.895
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.037	0.467
Rmeas	0.045	0.579
Rpim	0.026
Number of reflections	43344	2384
<I/σ(I)>	21.3	2.2
Completeness [%]	95.0	98.1
Redundancy	2.7	2.6
CC(1/2)	0.999	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.9	294.15	0.2M potassium nitrate, pH 6.9, 20% polyethylene glycol 3350