6A8K
Crystal structure of Ice-binding Protein from a Sea-Ice Microalga
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2015-06-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 36.054, 47.586, 134.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.590 - 1.400 |
| R-factor | 0.13806 |
| Rwork | 0.137 |
| R-free | 0.16220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vn3 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.478 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.057 | 0.434 |
| Number of reflections | 46394 | |
| <I/σ(I)> | 24.1 | 6.3 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 12.3 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl pH8.5, 2.0M Ammonium dihydrogen phosphate |
