6A8H
Crystal structure of endo-arabinanase ABN-TS D27A mutant in complex with arabinotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-21 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.125, 77.032, 88.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.350 - 1.650 |
| R-factor | 0.14944 |
| Rwork | 0.147 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wl7 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.019 | 0.178 |
| Number of reflections | 33864 | 1677 |
| <I/σ(I)> | 34.4 | 3.95 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 9.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 8000, 0.2M magnesium chloride, 0.1M Tris-HCl pH 8.5 |
