6A7X

Rat Xanthine oxidoreductase, D428A variant, NAD bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	98.135, 137.792, 222.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.630 - 2.150
R-factor	0.19807
Rwork	0.195
R-free	0.25488
RMSD bond length	0.009
RMSD bond angle	1.339
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.630	41.630
High resolution limit [Å]	2.150	2.150
Number of reflections	306011
<I/σ(I)>	1.72
Completeness [%]	99.4
Redundancy	1.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG 8000, HEPES, lithium sulfate, pH 6.2