6A7U

Crystal structure of histone H2A.Bbd-H2B dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	61.640, 141.910, 56.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.390 - 2.600
R-factor	0.2367
Rwork	0.235
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afa
RMSD bond length	0.010
RMSD bond angle	1.429
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.094	0.696
Number of reflections	8011	959
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	6.3
CC(1/2)	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1M sodium citrate tribasic dihydrate pH5.5 38% PEG 200