6A7S
Ca2+-independent C-type lectin SPL-2 from Saxidomus purpuratus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-05-19 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.235, 91.235, 48.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.720 - 2.000 |
| R-factor | 0.18079 |
| Rwork | 0.179 |
| R-free | 0.20745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.784 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.462 |
| Number of reflections | 16065 | |
| <I/σ(I)> | 39.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.2 M lithium sulfate, 0.1 M Tris-HCl (pH8.5), 30% PEG4000, 100 mM GalNAc, 10 mM CaCl2 |
