6A6Y

Crystal Structure of Asf1 from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-09-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	62.733, 62.733, 98.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.359 - 2.491
R-factor	0.1933
Rwork	0.192
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hue
RMSD bond length	0.003
RMSD bond angle	0.579
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.360	2.580
High resolution limit [Å]	2.491	2.491
Rmerge	0.123
Rmeas	0.132
Rpim	0.048
Number of reflections	13132	1258
<I/σ(I)>	13.86
Completeness [%]	97.8	95.66
Redundancy	7.5	7.3
CC(1/2)	0.997	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	30% PEG 3350, 0.2M Sodium thiocyanate, 0.1M MES pH 6.5