6A6W
Crystal structure of fission yeast inner membrane protein Bqt4 in complex with Sad1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 133.951, 133.951, 87.394 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.846 - 2.601 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ybx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.795 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.115 | 0.054 | 0.712 |
| Rmeas | 0.120 | 0.057 | 0.742 |
| Rpim | 0.033 | 0.017 | 0.207 |
| Total number of observations | 184255 | ||
| Number of reflections | 14717 | 845 | 721 |
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 12.5 | 10.3 | 12.8 |
| CC(1/2) | 0.999 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.4M Sodium malonate, pH7.0 |
