6A6W

Crystal structure of fission yeast inner membrane protein Bqt4 in complex with Sad1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97775
Spacegroup name	P 63 2 2
Unit cell lengths	133.951, 133.951, 87.394
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.846 - 2.601
R-factor	0.1837
Rwork	0.182
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybx
RMSD bond length	0.005
RMSD bond angle	0.795
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.115	0.054	0.712
Rmeas	0.120	0.057	0.742
Rpim	0.033	0.017	0.207
Total number of observations	184255
Number of reflections	14717	845	721
<I/σ(I)>	6.2
Completeness [%]	100.0	99.6	100
Redundancy	12.5	10.3	12.8
CC(1/2)		0.999	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	2.4M Sodium malonate, pH7.0