6A6V
Crystal structure of the modified fructosyl peptide oxidase from Aspergillus nidulans with 7 additional mutations, in complex with FSA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 |
Unit cell lengths | 135.540, 135.540, 144.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.457 - 2.900 |
R-factor | 0.1678 |
Rwork | 0.166 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a6r |
RMSD bond length | 0.007 |
RMSD bond angle | 1.477 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.130 | 0.852 |
Number of reflections | 33508 | 3301 |
<I/σ(I)> | 14 | |
Completeness [%] | 99.9 | |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 5% PGA, 20% PEG 3350, 0.1M CAPS (pH 8.8) |