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6A6L

Crystal structure of the cold shock domain of YB-1 in complex with m5C RNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL18U1
Synchrotron siteSSRF
BeamlineBL18U1
Temperature [K]100
Detector technologyCCD
Collection date2018-04-04
DetectorADSC QUANTUM 315
Wavelength(s)0.97930
Spacegroup nameP 62
Unit cell lengths66.380, 66.380, 34.702
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution29.709 - 1.780
R-factor0.1826
Rwork0.180
R-free0.20680
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3pf5
RMSD bond length0.006
RMSD bond angle0.934
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.8.4_1496)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0001.840
High resolution limit [Å]1.7801.780
Rmerge0.0700.191
Number of reflections8518856
<I/σ(I)>67.973
Completeness [%]100.0
Redundancy17.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2892.4M Ammonium sulfate

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