6A6L
Crystal structure of the cold shock domain of YB-1 in complex with m5C RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97930 |
Spacegroup name | P 62 |
Unit cell lengths | 66.380, 66.380, 34.702 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.709 - 1.780 |
R-factor | 0.1826 |
Rwork | 0.180 |
R-free | 0.20680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pf5 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.934 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.840 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.070 | 0.191 |
Number of reflections | 8518 | 856 |
<I/σ(I)> | 67.973 | |
Completeness [%] | 100.0 | |
Redundancy | 17.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2.4M Ammonium sulfate |