6A6H

Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-02-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	101.802, 101.802, 73.455
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	88.160 - 2.310
R-factor	0.2029
Rwork	0.199
R-free	0.27605
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h94
RMSD bond length	0.020
RMSD bond angle	2.033
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.160	2.500
High resolution limit [Å]	2.310	2.310
Rmerge	0.015	0.099
Number of reflections	19686	19686
<I/σ(I)>	22.96
Completeness [%]	89.9
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium acetate pH4.6 25% polyethylene glycol 4,000