6A6F

Crystal structure of Putative iron-sulfur cluster assembly scaffold protein for SUF system (FiSufU) from thermophilic Fervidobacterium Islandicum AW-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 1 2 1
Unit cell lengths	64.873, 30.665, 77.955
Unit cell angles	90.00, 98.65, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.16615
Rwork	0.163
R-free	0.22637
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qq4
RMSD bond length	0.014
RMSD bond angle	1.603
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.047	0.092
Number of reflections	18186
<I/σ(I)>	24.96
Completeness [%]	98.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.01M Nickel Chloride, PEG MEM 2000, 0.1M Tris HCl pH 8.5