6A68
the crystal structure of rat calcium-dependent activator protein for secretion (CAPS) DAMH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 149.305, 149.305, 81.414 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.884 - 2.901 |
| R-factor | 0.2574 |
| Rwork | 0.256 |
| R-free | 0.28620 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.740 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.127 | 0.828 |
| Rpim | 0.041 | 0.276 |
| Number of reflections | 12274 | |
| <I/σ(I)> | 16.8 | 2.4 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 9.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18-25% (v/v) PEG 3350, 0.1M Succinic acid (pH 7.0-7.5) |
