6A67
Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody FLD21.140
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 168.106, 168.106, 147.188 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.913 - 2.330 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dut |
RMSD bond length | 0.009 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.091 | 0.995 |
Number of reflections | 89876 | 4350 |
<I/σ(I)> | 22.3 | |
Completeness [%] | 99.5 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 2.0M (NH4)2SO4. 0.1M Bis-Tris pH 5.5 |