6A5Q
Structure of 14-3-3 beta in complex with TFEB 14-3-3 binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-12 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.170, 85.170, 209.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.880 - 2.000 |
| R-factor | 0.1871 |
| Rwork | 0.186 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bq0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.805 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.106 | 0.686 |
| Number of reflections | 60151 | 5860 |
| <I/σ(I)> | 24.9 | 4.7 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 11.6 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 289 | 0.1 M sodium malonate, and 12% (w/v) PEG 3350 |
