6A5M
Crystal structure of Arabidopsis thaliana SUVH6 in complex with SAM, form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.546, 77.098, 66.820 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 38.549 - 2.301 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a5k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.123 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.117 | 0.525 |
| Number of reflections | 24000 | 1223 |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M di-Sodium tartrate, 20% PEG 3350 |
