6A5G

The structure of [4+2] and [6+4] cyclase in the biosynthetic pathway of streptoseomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 2 21
Unit cell lengths	52.109, 85.023, 134.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.604 - 2.300
R-factor	0.1849
Rwork	0.180
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a5f
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.604	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.199
Number of reflections	24828
<I/σ(I)>	20.93
Completeness [%]	90.7
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295.15	2 M ammonium sulfate, 100 mM MES, pH 6.5, 200mM NaCl