6A5E
Crystal structure of plant peptide RALF23 in complex with FER and LLG2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 62.312, 62.360, 97.478 |
Unit cell angles | 105.72, 91.30, 108.48 |
Refinement procedure
Resolution | 42.607 - 2.766 |
R-factor | 0.2351 |
Rwork | 0.233 |
R-free | 0.28460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.298 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.830 |
High resolution limit [Å] | 2.760 | 2.760 |
Number of reflections | 32854 | 3150 |
<I/σ(I)> | 20.89 | |
Completeness [%] | 97.2 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium malonate, 25% w/v Polyethylene glycol 3350 |