6A5C

Crystal structure of plant Receptor-like Kinase ANX2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	123.374, 41.329, 93.366
Unit cell angles	90.00, 118.02, 90.00

Refinement procedure

Resolution	37.966 - 1.680
R-factor	0.1868
Rwork	0.186
R-free	0.20440
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.680	1.680
Number of reflections	47684	6472
<I/σ(I)>	36.4
Completeness [%]	99.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M sodium fluoride, 25% Polyethylene glycol 3350