6A5B

Crystal structure of plant Receptor-like Kinase FER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	60.545, 63.509, 107.776
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.595 - 2.205
R-factor	0.1937
Rwork	0.192
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a5c
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	21471	2099
<I/σ(I)>	20.4
Completeness [%]	98.2
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Bis-Tris pH 6.5, 25%(w/v) Polyethylene glycol 3350