6A5A
Crystal structure of plant Receptor-like Kinase ANX1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.337, 77.546, 99.288 |
Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
Resolution | 41.860 - 2.942 |
R-factor | 0.1996 |
Rwork | 0.196 |
R-free | 0.26040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a5c |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 2.940 | 2.940 |
Number of reflections | 19284 | 1750 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 98.3 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M ammonium acetate, 0.1 M BIS-Tris pH 6.5, 25%(w/v) Polyethylene glycol 3350 |