6A58

Structure of histone demethylase REF6

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	62.494, 62.494, 43.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.570
R-factor	0.21199
Rwork	0.211
R-free	0.23384
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Number of reflections	23425	1174
<I/σ(I)>	76.8
Completeness [%]	99.7	99.1
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG