6A4Z
Oxidase ChaP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.830, 47.120, 53.920 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 50.796 - 1.700 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.20690 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.800 | 50.800 | 1.730 |
| High resolution limit [Å] | 1.700 | 8.990 | 1.700 |
| Rmerge | 0.104 | 0.076 | 0.915 |
| Rmeas | 0.121 | 0.089 | 1.068 |
| Rpim | 0.060 | 0.045 | 0.538 |
| Total number of observations | 104844 | 709 | 5002 |
| Number of reflections | 27459 | 198 | 1373 |
| <I/σ(I)> | 8.1 | 19.3 | 1.5 |
| Completeness [%] | 97.3 | 96.6 | 92.4 |
| Redundancy | 3.8 | 3.6 | 3.6 |
| CC(1/2) | 0.993 | 0.986 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295.15 | MgCl2, Tris-HCl, PEG 4000 |
