6A4M

Structure of urate oxidase from Bacillus subtilis 168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.978
Spacegroup name	P 62 2 2
Unit cell lengths	133.050, 133.050, 90.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.2252
Rwork	0.223
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wlv
RMSD bond length	0.007
RMSD bond angle	1.144
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.162	0.064	1.068
Rmeas	0.164	0.065	1.083
Rpim	0.028	0.011	0.177
Number of reflections	15004	846	722
<I/σ(I)>	2.9
Completeness [%]	99.8	98	100
Redundancy	33.6	26.9	35.6
CC(1/2)		0.999	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl, 0.2 M NaAc.3H2O, 24% w/v PEG4000, pH 8.12