6A4F

Separated uridine bound Oligoribonuclease (ORN) from Colwellia psychrerythraea strain 34H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2017-03-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 32 2 1
Unit cell lengths	59.285, 59.285, 234.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.020 - 2.210
R-factor	0.19674
Rwork	0.193
R-free	0.27018
RMSD bond length	0.016
RMSD bond angle	1.884
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.210	2.210
Number of reflections	21675
<I/σ(I)>	43.9
Completeness [%]	86.2
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium thiocyanate, 20% (w/v) PEG 3350