6A4E
Two linked uridine bound Oligoribonuclease (ORN) from Colwellia psychrerythraea strain 34H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2018-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.667, 59.667, 236.201 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.340 - 2.450 |
R-factor | 0.239 |
Rwork | 0.237 |
R-free | 0.27580 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.204 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 18200 | |
<I/σ(I)> | 76.1 | |
Completeness [%] | 96.2 | |
Redundancy | 21.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.04 M citric acid, 0.06 M Bis-Tris propane pH 6.4, 20% (w/v) PEG 3350 |