6A4E

Two linked uridine bound Oligoribonuclease (ORN) from Colwellia psychrerythraea strain 34H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2018-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	59.667, 59.667, 236.201
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.340 - 2.450
R-factor	0.239
Rwork	0.237
R-free	0.27580
RMSD bond length	0.008
RMSD bond angle	1.204
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Number of reflections	18200
<I/σ(I)>	76.1
Completeness [%]	96.2
Redundancy	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	0.04 M citric acid, 0.06 M Bis-Tris propane pH 6.4, 20% (w/v) PEG 3350