6A4D

pNP-TMP bound Oligoribonuclease (ORN) from Colwellia psychrerythraea strain 34H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2016-11-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	59.532, 59.532, 236.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.250 - 2.190
R-factor	0.20806
Rwork	0.205
R-free	0.27064
RMSD bond length	0.020
RMSD bond angle	1.965
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.190	2.190
Number of reflections	25387
<I/σ(I)>	38.5
Completeness [%]	97.8
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.4 M ammonium tartrate dibasic, 0.1 M Tris pH 8.5