6A4A
Oligoribonuclease (ORN) from Colwellia psychrerythraea strain 34H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2016-06-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 53.112, 53.112, 143.338 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.521 - 2.700 |
R-factor | 0.2575 |
Rwork | 0.255 |
R-free | 0.29740 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.129 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.110 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 6155 | |
<I/σ(I)> | 56.6 | |
Completeness [%] | 99.8 | |
Redundancy | 22.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% (w/v) PEG 3350, 0.1 M magnesium formate |